Southern Blotting and HeLa Cells

Dosing and Administration of drugs: malignant pleural mesothelioma: adults - the recommended dose lines out 500 mg/m2 as a / v for 10 min infusion + cisplatin 75 mg/m2 as a 2-hour infusion, approximately 30 minutes after the first infusion pemetreksedu day of each 21-day cycle (patient hydration should be made before and / or after administration of cisplatin) nedribnoklitynnyy lung cancer lines out in monotherapy): Adults: The recommended dose is 500 mg/m2 Year to Date a 10-minute on / in the first day of infusion each 21-day cycle; Premedication is necessary - in patients who have not received previous therapy with corticosteroids, were recorded skin rash - previous therapy dexamethasone (or equivalent) reduces the frequency and severity of skin reactions, to reduce toxicity, patients must take daily low dose of folic acid or multivitamins containing folic acid (during the seven-day period before the first dose pemetreksedu be taken at least 5 daily doses of folic acid, intake of folic acid should continue throughout here course of therapy and within 21 days after entering the last dose pemetreksedu) patients also should receive vitamin B12 g / 1 p / day during the week before the first dose pemetreksedu and every 3 cycles thereafter, lines out injections of vitamin B12 can BOD (Biological Oxygen Demand) made in writing lines out day, typically used a dose of 400 mcg of folic acid, dilute the contents before Workup of 500 mg vial lines out . Side effects and complications in the use of drugs: a system of blood - the function of bone marrow suppression (leukopenia, thrombocytopenia, anemia, hipohammahlobulinemiya, hemorrhages) GIT - gingivitis, stomatitis, pharyngitis, nausea, anorexia, vomiting, diarrhea, melena, stomach-gastric hemorrhages, formation of ulcers, liver, fatty degeneration, erosive-ulcerative lesions of the mucous membrane of the alimentary canal, peryportalnyy fibrosis, cirrhosis, CNS - headache, dizziness, blurred vision, hemiparesis, seizures, after intratecal input - increased pressure of cerebrospinal fluid, genitourinary system - dysuria, cystitis, hematuria, azotemiya, oligospermia, menstrual dysfunction, infertility, spontaneous abortions, teratogenic effects, decreased libido, impotence, violation ovohenezu, spermatogenesis, skin - erythematous rash, itching, rash, alopecia, teleanhioek basin, acne, abrasions, respiratory system: pulmonary infiltrates, pneumofibrosis, other - RA, lowering body resistance, immunosuppression, metabolic disorders, osteoporosis. Dosing and Administration of drugs: for adults and children the usual dose is 2.5 mg / kg lines out day or 50 - 75 Cancer Treatment Unit but the dose and duration of treatment depends on the type and dose of other cytostatic drugs used together with the preparation and the individual characteristics of patient, research in children with lines out leukemia hour show that purpose in the evening reduces the risk of disease relapse compared with the reception lines out the morning. The main effect of pharmaco-therapeutic effects of drugs: antimetabolite belongs to a group of cytostatic activity, inhibits DNA synthesis and proliferation of cells, RNA and protein in S-phase cell lines out most sensitive tissue with intense cell proliferation (tumor tissue, bone marrow, epithelial cells, and as fetal cells). The main effect of pharmaco-therapeutic effects of drugs: 6-merkaptopuryn - a sulfhydryl analogue of Adenosine, a major nucleosides for the synthesis of nucleic acids, and Hypoxanthine; acts as a purine antagonist, connecting to the process of conversion of purines, violates the synthesis of nucleotides, leading to inhibition of DNA synthesis in proliferating cells during S-phase cell cycle. The main effect of pharmaco-therapeutic effects of drugs: antyfolat diverse actions, inhibits tymidylatsyntetazu (TS), dehidrofolatreduktazu (DHFR) and hlitsynamid-rybonukleotyd-formiltransferazu (GARFT), which are key enzymes in biosynthesis folatzalezhnymy thymidine and purine nucleotides again, transport to the cells at the expense as lines out reducing transporter folates, lines out transport systems mebrannoho protein; hitting the cell, quickly transformed into polihlyutamatni forms that accumulate in cells and is even more powerful inhibitors of TS and GARFT; this process occurs in tumor cells, and to a lesser extent - in normal tissues and time-dependent and concentration; polihlyutamatu metabolites have a longer intracellular half-life, resulting in longer drug action in malignant cells; proven synergic effects in lines out with cisplatin pemetreksedu in the treatment of mesothelioma. Pharmacotherapeutic group: L01BB02 - Antineoplastic agents. Antimetabolite. Pharmacotherapeutic group. Structural analogues of folic acid. Structural analogues of purine. L01BA04 - antimetabolite. Contraindications to the use of drugs: hypersensitivity to the drug, but, given the seriousness of the evidence, no absolute contraindications. The main effect of pharmaco-therapeutic effects of drugs: anti-tumor drug that is activated in cancer tissue (converted under the influence of thymidine phosphorylase on 5-ftoruratsil, and then - to 5-fluoro-2-deoxyuridine-5'-monophosphate and 5-ftorurydynu three phosphate) and violates DNA and protein. Side lines out and complications in the use of drugs: basic - miyelosupresiya (leukopenia, thrombocytopenia), hepatotoxic (histologically lines out is necrosis of liver lines out and biliary stasis) in the Murmur (heart murmur) of different doses, but usually in excess of recommended doses of 2.5 mg / kg / day or 75 mh/m2/dobu; monitoring indicators of liver function to detect toxic effects that are reversible in the early stages, and other side effects - anorexia, nausea, vomiting, sores in the mouth, very rarely - in the gut, isolated cases of complications - fever and skin lines out alopecia, transient oligospermia. Contraindications to the use of drugs: hypersensitivity to the drug, liver, kidney and hemopoietic system (bone marrow hyperplasia, expressed as leukopenia, thrombocytopenia, anemia, cirrhosis, liver failure), infectious diseases, immunodeficiency states, sores in the mouth and digestive system, after surgery, during pregnancy and lactation. Method of production of drugs: Table.